Software Release Notes

eProtein Discovery™ Instrument and Software

Release notes

Introduction

This document describes the software updates and improvements to:

eProtein Discovery Instrument Software
eProtein Discovery Experiment Workflow versions
eProtein Discovery software (Cloud-based)

Workflow versions can be identified when users start designing a new experiment. Release versions and description of change will be updated on this document periodically.

softwareDiagram

The above figure describes the eProtein Discovery™ software, instrument software and cloud-based architecture.

List of Releases:

September 2024 releases

New workflow release: eProtein Discovery™ Screen Experiment 5.1.0

⚠ This workflow will only be available on instruments that has been updated to software version 4.6.2. The following features will not be available retrospectively.

A new “Data Analytics” tab has been added that display charts of the experiment results

eProtein Discovery™ (cloud) software release

Users now have the capability to bulk import protein sequences from a fasta file by clicking on the + New Protein icon. This enhancement is particularly beneficial for users looking to import 24 sequences or more at once.

New status icons have been introduced in the "protein tab," allowing users to quickly view and check run status of each protein without needing to navigate to individual protein page for details.
Experiment can now be duplicated and renamed
Improved validation of DNA synthesis complexity:
- Complexity score with IDT recommendation will be displayed in full
- A warning will be presented if DNA synthesis complexity is above a threshold of 10
- High complexity AA sequences are blocked from proceeding to the next step
Protein sequences can now be exported in csv/fasta format
Improved eGene normalization and sequence calculation in the “Possible Constructs” tab
Addition of support links, bold, italics etc to notes field in proteins, projects and experiments
Amino acid sequences for all possible constructs are now available in the “Possible Construct” table
Protein sequences can now be exported in csv or fasta format by clicking on the ellipsis at the end of the protein list

Several bug fixes to improve user experience

July 2024 releases

Instrument software release version 4.6.2

Users can now export data to their SMBv3 network share on the local network directly from the instrument.
USB exports have been improved to show export progress
Experiments on online instruments are now automatically deleted after 30 days (only if all run data has been uploaded to the cloud), users can also manually delete experiments to free up disk space
Various bug fixes and stability improvements

June 2024 releases

eProtein Discovery (cloud) software release

Improvements to protein entry:
- DNA synthesis compatibility checks are more robust
Several improvements to the “Results” and “Scale-up Data” tabs, which will be available for experiments created using the workflow “eProtein Discovery Screen Experiment 4.0.0”
- In the Expression Yield Result table in the Results tab, expression conditions that were selected for purification are indicated with a blue tick
- eGene recipes that include the 3C protease additive are now annotated with a scissor icon in measurement tables
- The Scale-up Data tab now features an interactive graph which allows users to visualize predicted yields of constructs which were screened and purified.
- Constructs and conditions for scale up can be added to the Scale-up table by selecting from the graph. Scale-up volume and predicted yield can be adjusted to meet desired yield. The data from this table generates a downloadable .csv with calculated volumes to facilitate scale up set up at the bench.
Bug fixes:
- The “Possible constructs” tab is only visible after a protein is finalized
- The “Archive” button is re-positioned for improved visibility
- Any runs which were created with a protein that was later archived will have a warning when they get finalized

Instrument software release version 4.6.0

AI/ML-based automated droplet quality checks enabled. This feature will improve droplet quality checks and flag any compromised reactions to the user
Process improvements:
- PDF generation and report now downloadable straight from the instrument
- USB Export function now enabled
- Increased system memory stability
Bug fixes
- Automated Workflow version checks to ensure compatibility
- Optimized CPU usage
- Fixed issue occasionally observed due to rapid back to back runs
- eProtein Discovery Instrument software / Firmware interface with improved logging
Offline instrument (only for instruments installed to run in offline mode):
- Generic run can be created on the instrument without connection to the cloud software
  - Constructs and cell-free blend will be differentiated with a standard naming convention and cannot be customized on screen
  - Generic downselection of 30 highest expressing construct to the purification round
- USB only data export functionality enabled

New workflow release: eProtein Discovery Screen Experiment 4.0.0

⚠ The workflow version will only be available once the instrument software has been updated to version 4.6.0. The following features will not be available retrospectively.

Warnings are now displayed for measurements that may have been affected by droplet operation issues
A Transfer Plate Guide can now be downloaded from the experiment's Progress tab

New workflow release: eProtein Discovery Screen Experiment 4.0.1

eProtein Discovery Screen Experiment 4.0.1 now replaces eProtein Discovery Screen Experiment 4.0.0 with the following updates:

Fixed an issue where the "Top combinations for purified yield" table showed incorrect values

March 2024 releases

Instrument software release version 4.5.2

Implementation of pin-based login and logout screen.
Password change and reset can be done on the eProtein Discovery Software by clicking on the user icon on the top right of the screen. Once changed, new pin will automatically apply to the instrument login screen

Other improvements:

- Better cartridge serial number detection and identification
- Cartridge actuation to increase droplet dispensing accuracy

New workflow version release: eProtein Discovery Screen Experiment 3.0.0

⚠ The new workflow version will only be available once the instrument software has been updated to version 4.5.2. The following features will not be available retrospectively.

Added "Scale-Up Data" tab on the software for uploading scale-up data to completed experiments
A PDF report is now included in the ZIP file on the instrument result screen and eProtein Discovery software
Fixed bug that caused intermittent run failures

eProtein Discovery (cloud) software release

Users can now archive a protein, an experiment or a project generated on the eProtein Discovery Software (Retrieval of archived information is only available through Nuclera Technical Support).

Software release notes are now available for access at any time, under the “help” menu.
User feedback channel is now live, accessible through the “Help” menu.