ON-DEMAND WEBINAR

Defying Difficult-to-Express Proteins:

Overcoming Protein Expression Challenges

Introduction:

In this webinar, we will explore how the eProtein Discovery™ System addresses the challenges of expressing difficult-to-express proteins, such as transcription factors, proteins with disordered regions and disulfide bonds. Leveraging advanced cell-free protein synthesis (CFPS) and digital microfluidic technology, this system enables researchers to rapidly produce soluble, purified proteins within 48 hours. Using case studies, we will demonstrate how insights from expression and purification screening can be used to guide scale up protein production in cell-based systems. This seamless transition allows for the production of functional proteins from microgram/sub-milligram to milligram quantities. This webinar will illustrate how this technology revolutionizes protein expression, accelerating therapeutic development and advancing protein research.

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key points covered in this webinar:
  • Accelerating Protein Production: The eProtein Discovery™ system enables rapid, automated screening and production of difficult-to-express proteins, resulting in soluble, functional proteins within just 48 hours.
  • Case Studies: Real-world examples showcasing the successful expression of challenging proteins, such as transcription factors, through cell-free protein synthesis (CFPS).
  • From Cell-Free to Cells: Demonstrating how cell-free expression insights can be applied to cell-based systems, enabling scalable protein production from microgram/sub-milligram to milligram quantities of functional proteins.

 

About eProtein Discovery

Nuclera's eProtein Discovery™ system combines digital microfluidic droplet automation with cell-free protein synthesis technologies, it empowers protein scientists to identify the best conditions for expressing and purifying proteins of interest within 24 hours, all on a single consumable cartridge.

The system offers a significant advantage over traditional protein expression methods, allowing researchers to save time and resources by simplifying and automating the process. Its ability to handle multiple genes and customizable cell-free blends makes it a valuable tool for protein scientists in academia and the biopharma industry.