Resource Library

This webinar introduces the latest update to eProtein Discovery™, allowing researchers to customize cell-free expression conditions to improve yields of co-factor dependent membrane proteins.

In this flyer, we outline the 48-hour workflow to deliver protein in-hand including data on reproducibility, quality and quantity.

A comprehensive summarization of the eProtein Discovery System detailing its technical features and on which eGene kit to use for your individual needs. System specifications and ordering information is included.

Membrane proteins are the target of 60% of FDA-approved drugs, yet protein extraction and stabilization remain major challenges. eProtein Discovery™ overcomes these hurdles with cell-free protein synthesis, digital microfluidics, and optimized additive screening, delivering functional, correctly folded, and purified membrane proteins in just 48 hours. This poster highlights MsbA (an ABC transporter) and ZMPSTE24 (a…

Struggling with membrane protein expression? eProtein Discovery™ uses cell-free protein synthesis and automated additive screening to express and purify high-yield, assay-ready membrane proteins in just 48 hours. Optimize GPCR, ion channel, transporter and membrane-bound enzyme production with a streamlined workflow for cryo-EM, drug discovery, and functional assays.

Screen, Scale, Capture, Characterize – DNA to Protein Characterization in One Week Understanding drug–target interactions is essential to improve leads, optimize pharmacokinetics and predict in vivo efficacy. The process of generating soluble, functional drug targets and studying their interactions with drugs can be complicated and time-consuming, with multiple repeats needed to optimize protein expression and…

The Custom Additives manual covers how users can customize cell-free blends with additives of their own choice. This increases the flexibility for optimizing protein expression, folding and purification, by using user-chosen additives that are better tailored for their specific protein requirements while maintaining the automation and reproducibility provided by the eProtein Discovery™ system.

This application note showcases the eProtein Discovery™ system and the Custom Additive Initiative in addressing DHODH production challenges. By supplementing Cell-free Blends with custom additives such as FMN (a DHODH cofactor, essential for activity) and Brij®-35 (a surfactant known for stabilizing complex membrane proteins), conditions were identified to produce soluble, highly purified, and enzymatically active…

This Application Note, covers how we employed the eProtein Discovery System to successfully express and purify full-length Transcription Factor L (TF L), along with a domain of unknown function (L2) and its binding domain (L3).

This white paper investigates the cost of protein expression and the value delivered by the eProtein Discovery™ workflow. Traditionally, finding suitable conditions to obtain soluble and purifiable recombinant proteins is difficult and time-consuming. Constrained by cost, labor and throughput, typically only a few protein expression conditions are explored, with hit-and-miss results and often requiring multiple…