Accelerating the journey to your protein with eProtein DiscoveryTM
Are you aiming to get active proteins? Would a rapid protein prototyping system help you?
Identifying and obtaining winning proteins is a challenge in the drug discovery workflow. eProtein DiscoveryTM empowers scientists to advance protein projects with confidence.
It puts the latest technology on your bench to automate and optimize construct design, soluble expression and purification, providing you with protein to test in your applications.
Workflow
Step 1: Design & Prep
Design, order and prepare DNA expression constructs
Step 2: Load & Screen
Automated screens to determine your path to soluble purifiable proteins
Step 3: Scale up and Go!
Scale up proteins at µg -and mg- scale
Four components.
One complete protein solution.
How does eProtein DiscoveryTM work?
24 constructs x 8 cell free expression systems = 192 unique expression conditions
In 48 hours, determine the winning expression conditions from solubility, purifiablility and purified yield data to then scale up and get protein in hand to test in your downstream applications.
Thought-to-protein workflow. Intuitive software. Robust data. The protein you need to move forward.
Confidently advance with soluble, active, purifiable proteins
Express a wide range of proteins
eProtein DiscoveryTM can produce a variety of different proteins as small as 18kDa up to 300kDa: cytokines, growth factors and kinases to name a few.
Data-driven journey to your protein
Automated reports identify the best construct and expression conditions for scale up: solubility, purifiablility and purified yield.
Screening results of VEGF
As the key mediator of angiogenesis, vascular endothelial growth factor (VEGF), is an appealing target for anticancer therapeutics. However, identifying and producing soluble, purifiable VEGF with the functionality required for specific downstream applications is challenging.
This shows actual fluorescence results on the smart cartridge which give an indication of soluble protein expression. The variation in fluorescence intensity relates to the level of soluble protein expression.
Demonstration of soluble purified VEGF protein
VEGF expressed using conventional CFPS in tubes (lane 8) was compared against VEGF expression using the eProtein Discovery™ (lane 9), an average purity of 100% was obtained.
Reagent only (lanes 1 and 7), together with green fluorescent protein (GFP) expressed using eProtein Discovery™ were employed as negative controls to verify the expression of VEGF.
What do our customers say?
"Nuclera's technology represents fresh approaches, which will improve cost and quality significantly."
Prof. George Church
Professor of Genetics at Harvard Medical School and Professor of Health Sciences and Technology at Harvard and the Massachusetts Institute of Technology (MIT)
A protein system like no other
Combining unique cell free expression systems, novel digital microfluidics, and robust screening data, eProtein DiscoveryTM empowers scientists to perform their own protein screening, characterization, and small-scale expression in-house within days.
With eProtein DiscoveryTM, time is on your side.
Test hypotheses efficiently, get answers quickly, and take protein projects forward with confidence.
Frequently Asked Questions
Our team would be happy to connect with you!
Set up a protein consultation today.
Protein yield depends on your specific protein of interest. Our system uses cell-free protein synthesis (CFPS) reagents to scale up your protein. On average we have seen that you can get μg to mg of protein depending on the amount of CFPS reagents used.
On a single cartridge, you can simultaneously screen for expression using 24 protein constructs against 8 cell-free formulations (Cell-Free Blends). This allows you to test 192 unique expression conditions at once. Data from the expression screen is then used to select 30 expression conditions for a purification screen on the same cartridge.
In addition to your DNA construct, the reactions include purified cellular machinery, cofactors, substrates, and energy systems required for in vitro protein production. The reactions are composed of a Cell-free Core reagent which can be expanded to 8 variations using different additives such as:
- Chaperones
- Co-factors (e.g. FAD/NAD/SAM)
- Co-enzymes (e.g. metal mix)
- Disulfide bond formation additives
