APPLICATIONS
Purified, Functional Membrane Proteins in 48 hours with eProtein Discovery™
Our customizable membrane stabilizing screening solution accelerates production, delivering purified protein in 48 hours—optimizing folding, stability, yield, and function.
Screen your way to stable membrane proteins
Over 60% of drug targets are membrane proteins, yet producing them remains one of the greatest challenges in biopharmaceutical research. Are you facing challenges with misfolding, aggregation or low yields?
eProtein Discovery™ empowers you to express and purify stable, functional membrane protein in just 48 hours. With automated digital microfluidics and customizable cell-free expression, effortlessly screen membrane stabilizing agents, cofactors, redox conditions, and more. Overcome traditional bottlenecks and accelerate target discovery, validation, and lead optimization.
Explore your path to stable, purified, active, assay-ready membrane protein today.
Multiplexed membrane protein screening
Screen 11 membrane proteins in 8 customizable, stabilizing cell-free expression conditions.
Automated & miniaturized
Digital microfluidics powers automated nano-droplet screening for 88 expression and purification datapoints.
Rapid detection & characterization
On-cartridge fluorescence-based assay detects only full-length, stable membrane proteins.
Assay-ready protein
Purified, assay-ready membrane protein available for cryo-EM and functional assays in 48 hours.
The eProtein Discovery Membrane Protein Workflow
With eProtein Discovery, membrane protein optimization and production is faster than ever. Design constructs, screen with customizable, membrane-stabilizing cell-free conditions using automated digital microfluidics, and scale up stable, active membrane proteins for any downstream application.
eProtein Discovery: Your path to membrane protein
Optimize membrane protein production
Screen your way to stable membrane proteins
Membrane protein production is challenging due to structural complexity and instability. Conventional methods often yield misfolded or inactive proteins, delaying research and drug development for months.
Nuclera’s system accelerates membrane protein production. Within 24 hours, screen constructs against fine-tuned, customizable expression environments containing membrane stabilizing additives, such as nanodiscs, detergents and co-factors. Tailor folding and stability parameters to reduce misfolding and accelerate downstream workflows.
From GPCRs and ion channels to transporters and enzymes, streamline production with optimized conditions, ensuring high-yield, purified, assay-ready membrane proteins for efficient downstream applications.
Screening results from the eProtein Discovery System.
Automated & miniaturized
Overcome protein stability challenges
Automated digital microfluidics enables efficient, hands-free workflows. Screen 11 membrane protein constructs across 8 customizable, cell-free conditions with varying membrane mimetics—generating 88 expression and purification datapoints in a single 24 hour run. Increase screening throughput to identify the ideal nanodiscs, detergents, or other stabilizing additives that promote proper folding, high yield, and membrane protein stability. The nano-droplet format reduces reagent consumption, saves time, and minimizes experimental variability.
The built-in, on-cartridge fluorescence assay ensures only full-length, stable proteins are quantified. Advanced imaging algorithms track nanodroplet behavior—detecting subtle fluorescence patterns, identifying aggregation zones, and distinguishing soluble fractions with high precision. This enables accurate, real-time QC and confident decision-making.
With eProtein Discovery™, you obtain stable, properly folded membrane proteins—ready for successful downstream applications and drug discovery.
Automated digital microfluidics powers 88 expression and 88 purification datapoints.
Stable & purified membrane proteins
Purified, stable protein in 48 hours
From DNA to assay-ready membrane protein in 48 hours.
Scale up expression and purification seamlessly using data from your screen to produce purified, assay-ready membrane protein (µg–mg quantities). Easily move from construct design to functional protein, without aggregation, precipitation, or iterative troubleshooting.
Yield predictions obtained from the digital microfluidics screen align with scale-up results. Need more protein? Simply scale up reagents proportionally to increase yield.
Reduce iterative optimization cycles and accelerate the path to structural insights and functional validation.
Example scale up of a purified, stable and concentrated ABC transporter produced using the eProtein Discovery System.
Assay-ready protein
Functional, cryo-EM ready membrane proteins
eProtein Discovery delivers stable, functional membrane proteins within 48 hours, ready for cryo-EM, activity assays, and rapid target validation.
By screening constructs with customizable cell-free conditions and using fluorescence-based detection, you avoid months of trial and error.
This transformative system accelerates early-stage target discovery and drug development, enabling confident protein identification, validation, and optimization with minimal hands-on time. Unlock faster insights and breakthroughs with eProtein Discovery.
Activity data and cryo-EM images of scaled up ABC transporter produced using the eProtein Discovery System.
Explore every membrane protein class
From GPCRs and ion channels to transporters and enzymes, here are examples of membrane proteins produced using the eProtein Discovery System. Ready to tackle challenging membrane proteins? Unlock your next breakthrough with eProtein Discovery!
What can the eProtein Discovery produce?
eProtein Discovery expresses proteins from a wide range of organisms—including human, animal, plant, bacterial, fungal, yeast, and viral sources. It supports diverse protein classes and sizes, including GPCRs, ion channels, transporters, enzymes, cytokines/growth factors, transcription factors, and more. As the technology continues to evolve, so does its ability to express even more protein types, expanding opportunities for discovery across both research and therapeutic applications.
