Resource Library Find supporting resources for our platform. Filter by Resource TypeApplication NoteBrochureFlyerPosterPrimary LiteratureTechnical NoteVideoWebinar Open Filter Poster Poster: Screen, Scale, Capture, Characterize: Rapid protein production and characterization of drug-target interactions This poster illustrates how researchers can rapidly progress from DNA to an expressed, purified, and characterized protein—demonstrated here with Bruton’s tyrosine kinase (BTK)—using the eProtein Discovery and Biacore SPR systems. Webinar Webinar: Defying Difficult-to-Express Proteins – Overcoming Protein Expression Challenges In this webinar, we will explore how the eProtein Discovery™ System addresses the challenges of expressing difficult-to-express proteins, such as transcription factors, proteins with disordered regions and disulfide bonds. Leveraging advanced cell-free protein synthesis (CFPS) and digital microfluidic technology, this system enables researchers to rapidly produce soluble, purified proteins within 48 hours. Video Video – How to Carry Out Band-Stab Procedure Part of the in-the-lab instructional video series Video Video – How to Set Up a Run Part of the in-the-lab instructional video series Video Video – Steps to remove bubbles introduced during sample loading Video Video – Best Practices for Sample Loading Part of the in-the-lab instructional video series Video Video – How to connect the Base Fluid line Part of the in the lab instructional video series Poster Poster: Enhancing solubility of protein targets: innovations in expression strategies using eProtein Discovery™ An overview of solubility tags and their effects on soluble protein expression, as well as the impact of tag removal, and how it is a necessary and a high-value capability. Application Note Application Note: Customizing expression conditions with additives to attain soluble, active proteins within the eProtein Discovery™ System This application note highlights examples where cofactors, redox regulators and chaperones were added during protein expression leading to increase purification yields or active protein, therefore demonstrating the importance of these additives in protein folding and function. Application Note Application Note: Rapid Cell-Free Expression and Purification of B-lymphocyte Antigen CD19 Using eProtein Discovery™ In this Application Note, we leverage the eProtein Discovery™ system to screen 192 expression conditions and optimize the production of soluble CD19 protein within 24 hours. We successfully expressed and purified three CD19 length variants: full-length CD19, the N-terminal extracellular domain, and the C-terminal intracellular domain, overcoming challenges associated with “difficult-to-express” proteins. Pages: 123Load More Resources