Resource Library

This Application Note, covers how we employed the eProtein Discovery System to successfully express and purify full-length Transcription Factor L (TF L), along with a domain of unknown function (L2) and its binding domain (L3).

This white paper investigates the cost of protein expression and the value delivered by the eProtein Discovery™ workflow. Traditionally, finding suitable conditions to obtain soluble and purifiable recombinant proteins is difficult and time-consuming. Constrained by cost, labor and throughput, typically only a few protein expression conditions are explored, with hit-and-miss results and often requiring multiple…

The eProtein Discovery System simplifies the transition from cell-free protein synthesis (CFPS) to in vivo. E. coli expression, especially for intricate proteins with disulfide bonds. By evaluating numerous protein variants and up to seven solubility tags, researchers can efficiently identify optimal construct designs.

This poster illustrates how researchers can rapidly progress from DNA to an expressed, purified, and characterized protein—demonstrated here with Bruton’s tyrosine kinase (BTK)—using the eProtein Discovery and Biacore SPR systems.

In this webinar, we will explore how the eProtein Discovery™ System addresses the challenges of expressing difficult-to-express proteins, such as transcription factors, proteins with disordered regions and disulfide bonds. Leveraging advanced cell-free protein synthesis (CFPS) and digital microfluidic technology, this system enables researchers to rapidly produce soluble, purified proteins within 48 hours.

An overview of eProtein Discovery™, a rapid protein access system located on your benchtop, which enables you to go from DNA to protein in less than 48 hours.

Nuclera has developed eGene™ Prep Kits to rapidly convert your Gene of Interest into expression cassettes that are immediately compatible with eProtein Discovery™ reagents for Cell-Free Protein Synthesis (CFPS). CFPS utilizes linear DNAs (eGene™ constructs) as one of its ingredients to power the cell-free machinery to produce protein.

Part of the in-the-lab instructional video series

Part of the in-the-lab instructional video series