Resource Library

This application note showcases how Nuclera’s eProtein Discovery™ system, combined with Cytiva’s Biacore™ SPR platform, overcomes major bottlenecks in kinase drug discovery by enabling rapid, high-yield production and kinetic analysis of Bruton’s Tyrosine Kinase (BTK) variants.

Join experts from IDT, Nuclera, and Aldevron for an exclusive webinar showcasing a fully integrated solution that takes you from gene to assay-ready protein in just days — not weeks.

This video showcases the seamless integration of AlphaFold into Nuclera’s eProtein Discovery™ Cloud software, enabling a powerful, structure-guided approach to protein engineering.

This application note highlights the capabilities of the eProtein Discovery™ system in overcoming the challenges associated with expressing and purifying the membrane protein MsbA, an ABC transporter integral to bacterial lipid transport. By leveraging the system’s automated, screening of DNA constructs and customizable cell-free blends, containing membrane mimetics, researchers successfully identified optimal conditions to produce…

Membrane proteins are the target of 60% of FDA-approved drugs, yet protein extraction and stabilization remain major challenges. eProtein Discovery™ overcomes these hurdles with cell-free protein synthesis, digital microfluidics, and optimized additive screening, delivering functional, correctly folded, and purified membrane proteins in just 48 hours. This poster highlights MsbA (an ABC transporter) and ZMPSTE24 (a…

An overview of how eProtein Discovery™ integrates AlphaFold2 to enable rapid and precise protein variant design. By leveraging 3D structural insights, researchers can identify key features and domains to optimize protein stability, solubility, and function within a streamlined workflow. Whether you're looking to improve your protein engineering strategies or optimize your research workflow, this resource offers valuable insights into automated protein design and expression.

This study integrates AlphaFold’s AI-based structure prediction with high-throughput screening on the eProtein Discovery™ system to optimize protein solubility. By designing protein variants based on AlphaFold predictions and screening their expression and purification, we significantly increase soluble protein yields. Case studies on MMP-1 and MDM2 demonstrate this workflow’s efficiency, accelerating protein production for structural biology and drug discovery applications within 48 hours.

This webinar introduces the latest update to eProtein Discovery™, allowing researchers to customize cell-free expression conditions to improve yields of co-factor dependent membrane proteins.

In this flyer, we outline the 48-hour workflow to deliver protein in-hand including data on reproducibility, quality and quantity.

A comprehensive summarization of the eProtein Discovery System detailing its technical features and on which eGene kit to use for your individual needs. System specifications and ordering information is included.