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Poster

Poster: Enhancing solubility of protein targets: innovations in expression strategies using eProtein Discovery™

An overview of solubility tags and their effects on soluble protein expression, as well as the impact of tag removal, and how it is a necessary and a high-value capability.

Application Note

Application Note: Customizing expression conditions with additives to attain soluble, active proteins within the eProtein Discovery™ System

This application note highlights examples where cofactors, redox regulators and chaperones were added during protein expression leading to increase purification yields or active protein, therefore demonstrating the importance of these additives in protein folding and function.

Application Note

Application Note: Rapid Cell-Free Expression and Purification of B-lymphocyte Antigen CD19 Using eProtein Discovery™

In this Application Note, we leverage the eProtein Discovery™ system to screen 192 expression conditions and optimize the production of soluble CD19 protein within 24 hours. We successfully expressed and purified three CD19 length variants: full-length CD19, the N-terminal extracellular domain, and the C-terminal intracellular domain, overcoming challenges associated with “difficult-to-express” proteins.

Webinar

On-demand Webinar: Predictions to Proteins In-Hand in just 48hrs – Rapidly Express AlphaFold Predictions

The integration of AlphaFold AI within the eProtein Discovery™ framework brings forth a multitude of advantages, facilitating structure guided protein construct design with unprecedented precision.

Webinar

On-demand Webinar: Intro to Rapid Protein Expression Automation with eProtein Discovery

The eProtein Discovery™ System can screen multiple protein expression and purification profiles and deliver reliable proteins in-hand in less than 48 hours. Revolutionizing laboratory workflows and making a demonstrable impact in terms of time to publish in academia and drug discovery timelines.

On-demand webinar Automated Protein Expression Series Intro to Rapid Protein Expression Automation with eProtein Discovery™

Flyer

Flyer: Protein in-hand in 48 hours: eProtein Discovery™

In this flyer, we outline the 48-hour workflow to deliver protein in-hand including data on reproducibility, quality and quantity.

Video

Video – Intro to eProtein Discovery™

An overview of eProtein Discovery™, a rapid protein access system located on your benchtop, which enables you to go from DNA to protein in less than 48 hours.

Poster

Poster: DNA to assay-ready proteins in 48 hours. Rapid protein expression and purification on the eProtein Discovery™ system and binding confirmation on Biacore™ SPR System

We show in this poster evidence from combining rapid protein obtainability on the eProtein Discovery™ system, and functional activity via protein-protein interaction studies on Biacore™ SPR system. This workflow is demonstrated using a difficult-to-express protein VEGF165, known to contain disulfide bonds.

Nuclera Poster VEGF165 Binding Confirmation Mockup Image

Webinar

On-demand Webinar: Overcoming the Inexpressible: How cell-free protein synthesis solved the E. coli toxicity hurdle

Overcoming the Inexpressible: How cell-free protein synthesis solved the E. coli toxicity hurdle. Learn how the eProtein Discovery™ enabled optimization of the expression conditions for an GCN5-Related N-Acetyltransferase protein and gain insights into its antibiotic resistance mechanism.

Brochure

eProtein Discovery™ construct design and expression optimization guide

This brochure covers how you can simultaneously screen 24 DNA constructs across 8 customizable Cell-free Blends, ensuring optimal protein yield and delivering results in just 48 hours. Explore how eProtein Discovery revolutionizes protein production with unparalleled speed and success.

Pages: 123

eProtein Discovery System

The only end-to-end protein prototyping solution

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