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Technical Note

Customize DNA constructs (eGene) with pre-set fusion tags to optimize protein expression

Nuclera has developed eGene™ Prep Kits to rapidly convert your Gene of Interest into expression cassettes that are immediately compatible with eProtein Discovery™ reagents for Cell-Free Protein Synthesis (CFPS). CFPS utilizes linear DNAs (eGene™ constructs) as one of its ingredients to power the cell-free machinery to produce protein.

Brochure

Brochure: eProtein Discovery™ Product Specification

A comprehensive summarization of the eProtein Discovery System detailing its technical features and on which eGene kit to use for your individual needs. System specifications and ordering information is included.

Application Note

Application Note: Screening the effects of solubility tags and their removal (detagging) with 3C protease on cell-free protein expression using the eProtein Discovery™ System

The ability to evaluate the effect of different solubility tags on protein expression and the impact of their removal can be done simultaneously on eProtein Discovery. Data in this application note validates the benefit of having a solubility tag during expression and confirming that removal of the solubility tag retains yield.