Customizing expression conditions with additives to attain soluble, active proteins within the eProtein Discovery™ System
This application note highlights examples where cofactors, redox regulators and chaperones were added during protein expression leading to increase purification yields or active protein, therefore demonstrating the importance of these additives in protein folding and function.
From this reading you'll get an appreciation of the following:
- View the 10 additives that are included in the eProtein Discovery Additive selection panel
- A summary of the additive options and their respective benefits to protein folding
- See a decision tree to guide users with additive selection
Contributors to Application Note
Thomas Guilliam - Senior Scientist Lysate Development.
Brittany Wall - Scientist (Protein).
Sina Schack - Scientist (Protein).
Filippo Caschera - Cell-free protein synthesis.
Download: Customizing expression conditions with additives to attain soluble, active proteins within the eProtein Discovery System
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