The Smart Cartridge - Enabling Pipette and ForgetTM within eProtein DiscoveryTM
Pipette reagents into the Smart Cartridge to get comprehensive data on protein solubility, purifiability and purifiable yield in a day!
Features and Benefits
Powered by digital microfluidic technology, software controlled digital signals guide the movement of droplets on the Smart Cartridge surface to enable splitting, dispensing and merging of biological reagents. Pipette DNA, cell free expression reagents and purification solutions on the Smart Cartridge and the technology will deliver the rest.
Gain precise control of your eGene™ constructs and reagents to screen and discover optimal expressing conditions within 24 hours, accelerating target selection. A simple set-up allows anyone to run the system with minimal training.
Microfluidic lab-on-a-cartridge
Automate separate workflows of expression, characterization and purification all onto one cartridge
Pipette and forgetTM
Pipette reagents for screen and purification and eProtein DiscoveryTM delivers the rest
192X Multiplex expression optimization
192X screening pairs analyzed in one run and then obtainable candidates selected for purification
Examples of proteins synthesized with eProtein Discovery™
Protein Class
Protein Name
- Interferon gamma (IFN-γ)
- Programmed cell death protein 1 (PD1)
- Erythropoietin
- Vascular endothelial growth factor (VEGF)
- Interleukin-2 (IL-2)
Technical Specifications
| Multiplex optimization | 24 DNA constructs x 8 conditions = 192 data points screened in parallel |
| Fluorescent end point detection capability | Fluorescent data for 192 sites on cartridge |
| Magnetic bead-based purification | Miniaturized protein purification on chip for purifiability assessment |
| High resolution digital droplet control | 250k individual pixels on cartridge enables precise droplet acrobatics |
| Electrowetting on dielectric Smart Cartridge | Digital signals to manipulate droplets of diverse chemistry |
| Protected by vacuum deposition smart coating technology | Coating optimized to enable precise movement of small volumes |
Featured Resource
On-demand Webinar: Intro to Rapid Protein Expression Automation with eProtein Discovery
The eProtein Discovery™ System can screen multiple protein expression and purification profiles and deliver reliable proteins in-hand in less than 48 hours. Revolutionizing laboratory workflows and making a demonstrable impact in terms of time to publish in academia and drug discovery timelines.
Customize DNA constructs (eGene) with pre-set fusion tags to optimize protein expression
Nuclera has developed eGene™ Prep Kits to rapidly convert your Gene of Interest into expression cassettes that are immediately compatible with eProtein Discovery™ reagents for Cell-Free Protein Synthesis (CFPS). CFPS utilizes linear DNAs (eGene™ constructs) as one of its ingredients to power the cell-free machinery to produce protein.
